Rapid discovery of self-assembling peptides with one-bead one-compound peptide library

Abstract Self-assembling peptides have shown tremendous potential in the fields of material sciences, nanoscience, and medicine. Because vast combinatorial space even short peptides, identification self-assembling sequences remains a challenge. Herein, we develop an experimental method to rapidly screen huge array peptide for property, using one-bead one-compound (OBOC) library method. In this approach, on beads are N-terminally capped with nitro-1,2,3-benzoxadiazole, hydrophobicity-sensitive fluorescence molecule. Beads displaying would fluoresce under aqueous environment. Using identify eight pentapeptides, all which able self-assemble into nanoparticles or nanofibers. Some them interact taken up efficiently by HeLa cells. Intracellular distribution varied among these non-toxic peptidic nanoparticles. This simple screening strategy has enabled rapid suitable development nanostructures various biomedical applications.